Nucleoside Triphosphate-Nucleoside Diphosphate Transphosphorylase (Nucleoside Diphosphokinase)
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چکیده
منابع مشابه
Nucleoside triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 3. Subunit structure of the crystalline enzyme from brewers' yeast.
Previous physical studies from this laboratory by sedimentation velocity and ditfusion and by sedimentation equilibrium (YuE, R. H., RATLIFF, R. L., AND KWY, S. A. (1967) Biochemistry 6, 2923-2932) have permitted an assignment of the kinetic molecular weight of the crystalline brewers’ yeast nucleoside diphosphokinase, namely 102,000 ( 12,000) at infinite dilution. The observed concentration de...
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Nucleoside diphosphokinase (NDP kinase) was identified in high concentrations in human erythrocytes, and a procedure was developed for the isolation of this enzyme. The best preparations had about 1400-fold greater specific activity (65 units per mg of protein) than that of hemolysates. Like crystalline yeast NDP kinase and partially purified NDP kinase preparations from various tissues, the er...
متن کاملErythrocytic Nucleoside Diphosphokinase
Nucleoside diphosphokinase (NDP kinase) was identified in high concentrations in human erythrocytes, and a procedure was developed for the isolation of this enzyme. The best preparations had about 1400-fold greater specific activity (65 units per mg of protein) than that of hemolysates. Like crystalline yeast NDP kinase and partially purified NDP kinase preparations from various tissues, the er...
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Nucleoside diphosphokinase from Salmonella typhimurium has been purified and characterized. In many respects, the enzyme is similar to those from higher organisms in that it has a broad speciCcity for both phosphate donor and recipient and that it functions via a ping-pong mechanism. The enzyme activity of cells is very high at all growth rates but decreases as growth rate increases. Evidence i...
متن کاملAlpha-carboxy nucleoside phosphonates as universal nucleoside triphosphate mimics.
Polymerases have a structurally highly conserved negatively charged amino acid motif that is strictly required for Mg(2+) cation-dependent catalytic incorporation of (d)NTP nucleotides into nucleic acids. Based on these characteristics, a nucleoside monophosphonate scaffold, α-carboxy nucleoside phosphonate (α-CNP), was designed that is recognized by a variety of polymerases. Kinetic, biochemic...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43793-9